2023 AOSSM Annual Meeting Recordings with CME-Characterization of Bone in Osteochondral Allografts Utilizing Inflammatory-Mediated Cellular Death Pathways

Characterization of Bone in Osteochondral Allografts Utilizing Inflammatory-Mediated Cellular Death Pathways

Video Transcription

Video Summary

In this video, the speaker discusses the use of osteochondroallografts to treat large chondral defects. They mention that the survival rate at 15 to 20 years is around 75%, but failure is often associated with MRI cystic changes and persistent clefts caused by inflammation. The speaker explains that previous studies have primarily focused on cell death within the cartilage component of these grafts, but not within the bone component. They discuss different cell death mechanisms, including apoptosis, pyroptosis, necroptosis, and panoptosis, which are associated with activation of specific enzymes and proteins. The speaker then describes an experiment where fixed samples were used to analyze cell death and activation of inflammatory cell death pathways in the bone and cartilage components of osteochondroallografts. They find that cell death is significantly higher in the bone compared to the cartilage. Activation of pyroptosis, necroptosis, and apoptosis is observed in both components. However, no significant correlation is found between temperature or time and activation of these pathways. The speaker suggests that using a thin backing of bone and reducing the cellular component of the grafts may help decrease inflammation. They also propose future studies to investigate the potential of inhibitors of inflammatory cell death pathways in improving outcomes. The speaker acknowledges the contributions of Dr. Rodeo, Lily, and Krishna Anand.

Asset Caption

Mark T. Langhans, MD, PhD

Keywords

osteochondroallografts

chondral defects

cell death mechanisms

inflammatory cell death pathways

inhibitors of inflammatory cell death pathways